Credential Summary
| Credential Name | Shimadzu High Performance Liquid Chromatography (HPLC): Applied Method Development and Purification Scale-Up |
| Credential Type | Certificate of completion issued by the training provider, Walsh University. |
| Training Hours | 8 contact hours, delivered in one day of in-person, instructor-led training. |
| Training Provider | Certificate of completion issued by the training provider, Walsh University. |
| Cost per Participant | $1,000 per participant |
| How the Credential is Earned | Participants complete a practical check at the end of each of the four blocks, reviewed by the instructor, and attend the full 8 hours. Walsh University then awards the certificate of completion under the credential name shown above. |
Course Description
This course teaches laboratory technicians, analytical chemists, and quality control professionals to develop HPLC methods and scale them from analytical to preparative purification. Participants select columns and mobile phases, develop and optimize isocratic and gradient methods, run sample sequences in LabSolutions, build calibrations to quantify samples, determine column loading limits, and scale an analytical method to a preparative column. The course is a stand-alone program that can be taken on its own.
Detailed Training Outline
The day is built from short technical explanations, instructor demonstrations, and hands-on work that participants complete on Shimadzu HPLC systems with LabSolutions software during the day. No prerequisites are required.
1. Column and Mobile Phase Selection and Isocratic Development:
Technical objectives: confirm that the system is equilibrated with a stable baseline; select a column and mobile phase for a group of analytes; adjust mobile phase composition to control retention; develop an isocratic method.
Hands-on work: the instructor demonstrates the effect of composition changes on one mixture, then participants equilibrate their system, run the mixture at three compositions, record retention and resolution, and choose the best isocratic conditions.
Output: a record of runs and the selected conditions.
2. Gradient Development and Optimization:
Technical objectives: build a gradient program; adjust slope and starting and ending composition to improve resolution and shorten run time.
Hands-on work: the instructor builds a first gradient live, then participants modify gradient settings across several runs, compare resolution and run time, and select a final gradient.
Output: a final gradient method with a comparison of runs.
3. Sequences, Integration, Calibration, and Quantitation:
Technical objectives: set up and run a sequence with blanks, standards, and samples in LabSolutions; set integration parameters and identify peaks; create a multi-level calibration and evaluate linearity; quantify unknown samples with external and internal standards.
Hands-on work: the instructor demonstrates sequence setup and integration, then participants run a sequence, build a calibration, and quantify two unknown samples.
Output: a processed sequence with calibration and quantitation results.
4. Scaling from Analytical to Preparative Purification:
Technical objectives: perform a loading study to find the maximum injection load; calculate the preparative flow rate, gradient time, and injection load from the analytical method and the column dimensions; evaluate fraction purity and recovery with an analytical run; document the scaled method.
Hands-on work: the instructor demonstrates a loading study and a worked scale-up calculation, then participants run a loading study on the analytical column, calculate preparative conditions for a larger column, and evaluate purity and recovery from provided fraction data.
Output: a loading study record and a scaled preparative method with calculations.
Technical Learning Objectives
By the end of the course, participants will be able to:- Confirm that a system is equilibrated and stable before beginning method work
- Select a column and mobile phase for a given group of analytes and adjust mobile phase composition to control retention
- Develop and optimize isocratic and gradient methods to improve resolution and shorten run time
- Set up and run a sequence in LabSolutions that includes blanks, standards, and samples, and set integration parameters to identify peaks
- Create a multi-level calibration curve, evaluate linearity, and quantify unknown samples using external and internal standards
- Perform a loading study to determine the maximum injection load on an analytical column
- Scale an analytical method to a preparative column by calculating flow rate, gradient time, and injection load from the column dimensions
- Evaluate the purity and recovery of collected fractions with an analytical run, and document the method and results
Evidence the Skills Have Value Beyond One Employer
The skills taught in this course are not specific to any one employer. They are transferable across every industry that uses liquid chromatography, including pharmaceutical, biotechnology, food and beverage, environmental, chemical, and manufacturing laboratories, because the course teaches standard chromatographic practice (method development, calibration, quantitation, and scale-up from analytical to preparative purification) that applies to HPLC instruments from all manufacturers and to purification work across laboratories. O*NET lists chromatography and laboratory analysis among the work of Chemical Technicians. All hands-on work uses instructor-provided instruments, standards, and samples, not any organization’s materials.